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Human Lymphoid enhancer-binding factor 1 (a.a.1-399)

LEF1 Swiss Prot#

Q9UJU2

LEF1 Protein names

Lymphoid enhancer-binding factor 1 (LEF-1) (T cell-specific transcription

factor 1-alpha) (TCF1-alpha)

LEF1 Gene names

LEF1

Organism

Human

LEF1 Entry name

LEF1_HUMAN

LEF1 Codon optimized cDNA Clone

LEF1 CDNA Cat#

RPS0446

cDNA Tag

cDNA LEF1 Contains A 6x histidine tag

Condon optimized LEF1 CDNA Formulation

Liquid

Condon optimized LEF1 CDNA Storage

Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw

Condon optimized LEF1 cDNA Applications

Applications: 1. Codon-optimized cDNA sequence designed for recombinant

protein production in both E.coli and Mammalian cell. The cDNA has been

cloned into E.coli expression vector with 6x Histidine tag.2. May be used as

RNAi assistance to restore the loss-of-function.

Recombinant LEF1 Protein

Recombinant LEF1 Protein Cat#

PS0446

LEF1 Protein Source

Recombinant protein.

Recombinant LEF1 Protein Applications

Western Blot, may be used for other applications determined by user

Recombinant LEF1 Protein Purity

>90%, as determined by SDS-PAGE under reducing conditions

Recombinant LEF1 Protein Activity

N/A

Recombinant protein Tag

Recombinant LEF1 contains A 6x histidine tag

Recombinant LEF1 Protein Formulation

Liquid

Recombinant LEF1 Protein Storage

Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw

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LEF1 Protein family

TCF/LEF family

LEF1 Subcellular locations

Nucleus.

LEF1 FUNCTION (According to UniProtKB/Swiss Prot)

FUNCTION: Participates in the Wnt signaling pathway. Activates

transcription of target genes in the presence of CTNNB1 and EP300. May

play a role in hair cell differentiation and follicle morphogenesis. TLE1,

TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and

CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a

sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-

independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1

interaction domain and may be an antagonist for Wnt signaling. Isoform 5

transcriptionally activates the fibronectin promoter, binds to and represses

transcription from the E-cadherin promoter in a CTNNB1-independent

manner, and is involved in reducing cellular aggregation and increasing

cell migration of pancreatic cancer cells. Isoform 1 transcriptionally

activates MYC and CCND1 expression and enhances proliferation of

pancreatic tumor cells.

LEF1 Sequence databases

EMBL

DDBJ

GenBank

LEF1 3D structure databases

ModBase

LEF1 Protein-protein interaction databases

STRING

LEF1 Proteomic databases

PRIDE

LEF1 Genome annotation databases

Ensembl

GeneID

KEGG

UCSC

LEF1 Organism specific databases

CTD

MGI

LEF1 Phylogenomic databases

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

LEF1 Gene expression databases

Bgee

ArrayExpress

Genevestigator

LEF1 Family and domain databases

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY