Service
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Gene Synthesis (Cat# GS-101)
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- FAST TURNAROUNDTIME: 10-15 Business days
- OPTIMIZE CODON USAGE: Free
- QUALITY: 100% match with your requested sequences
- FLEXIBILITY: Free to clone the synthesized gene into any of your desired vector
- PRODUCTS: Final products (2 μg plasmid or glycerol stock with detail data sheet )will be delivered.
- LOWER PRICE: Call or email for quotation
- To place order, please download ">>>Gene synthesis order form" and Email to order@bioclone.us
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Recombinant Protein Production and Purification
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E.coli Expression System (Cat# RES-101)
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Phase I: Expression vector preparation
1. If customer provides cDNA
- Subcloning your interest cDNA into bacterial expression vector with His-tag or GST-tag or customer’s expressin vector.
- Mini-analysis of the expression of the gene in two different bacterial expression strains and three different temperature (16˚ C,
28˚ and 37˚C) by SDS-PAGE or Western blot to check protein solubility,stability and expression level.
- Estimated time: 5-10 Business days
- Price: $1,200
2. If customer does not provide cDNA
- Bioclone will artificially synthesize your interest cDNA , which will be codon-optimized for maximum expression (Normally,
expression level > 2-100 times higher than original cDNA). clone into expression vector.
- Mini-analysis of the expression of the gene in two different bacterial expression strains and three different temperature (16˚ C,28˚
and 37˚C) by SDS-PAGE or Western blot to check protein solubility,stability and expression level.
- Estimated time: 5-10 Business days
- Price: $1.45 /bp (if cDNA <500bp, $600 will be charge for set-up fee)
Phase II: Large-scale culture and purification
- Affinity purification from: 0.5 liter fermented bacterial culture/$590 (Purity>90%)
- Affinity purification from: 1.0 liter fermented bacterial culture/$890 (Purity>90%)
- Estimated time: 5 business days
Customer will get 2µg plasmid and recombinant protein and detail datasheet
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Plant Expression System (Cat# RPS-101)
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1. Send us your interest cDNA or protein sequence, we will send you purified His-tagged recombinant protein.
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sf9 Insect Cells Expression System (Cat# RIS-101)
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Phase I: Expression vector preparation
1. If customer provides cDNA
- Subcloning your interest cDNA into sf9 expression vector with His-tag and construct bacmid
- Price $2500
2. If customer does not provide cDNA
- Bioclon will optimize codons (codon optimization will dramatically increase expression level as much as > 2-100 times),
artificially synthesize cDNA, sequencing and cloning into sf9 expression vector with His-tag and construct bacmid.
- Estimated time: 2-3 weeks
- Price: $1000+$1.45/bp (if cDNA <1000bp, $600 will be charged for set-up fee)
Phase II: Mini analysis
- Transfection of sf9 insect cells with bacmid and Plaque purification and viral titer assay.
- Virus stock preparation
- Affinity protein purification from 500ml of culture (Purity >90% determined by SDS-PAGE)
- Estimated time: 4-6 weeks
- Price: $3500
Customer will get 2µg plasmid and Bacmid , purified recombinant and detail datasheet
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Transient Mammalian Expression in CHO-K1 or HEK293 (Cat# RMS-101)
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Phase I:
1.If customer provides cDNA
- Cloning of your interest gene into mammalian expression vector with His-tag
- Mini-scale (5ml) transfection to test the expression level and optimization.
- Estimated time: 3-4 weeks
- Price $2,500
2. If customer does not provide cDNA
- Bioclon will optimize codons (codon optimization will dramatically increase expression level as much as > 2-100 times), artificially
synthesize cDNA, sequencing and cloning into mammalian expression vector with His-tag.
- Mini-scale (5ml) transfection to test the expression level and optimization.
- Estimated time: 4-6 weeks
- Price: $1500+$1.45/bp (if cDNA <1000bp, $600 will be charge for set-up fee)
Phase II:
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Conjugation to Magnetic Beads (Cat# CMS-101)
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- Just send us your protein, or peptide, or DNA or Oligo-nucleotides or Antibody, we will conjugate them to our magnetic Beads.
- Price: 30 mg of magnetic beads/$990
- Turnaround time: 1 week
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Other Molecular Biology Service
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- For other molecular biology service, please call for quotation !
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