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- Very good matrix for coupling antibodies which has limited
amounts available-
- Conjugation over a broad pH range
- High coupling efficiency
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- Antigen-binding site in some antibodies
may be adversely affected by coupling.
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- Site-directed immobilization results in the best retention of
antibody-binding activity
- Ideal matrix for polyclonal antibody, especially for
antibodies with low avidity for antigen
- High coupling efficiency
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- Some monoclonal antibodies may lack
accessible carbohydrate for coupling.
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- Ideal matrix for reversibly immobilizing thiol-containing
ligands to allows for gentle elution conditions
- Site-directed immobilization results in the best retention of
antibody-binding activity
- Pre-activated, ready-to-use
- High coupling efficiency
- Allows for gentle elution conditions
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- Antibody has to be first reduced befor
coupling
- Not suitable for antibodies with low
affinity for their antigens
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- Excellent matrix for coupling antibodies having extremely
high avidity for their antigen
- Site-directed immobilization results in the best retention of
antigen-binding activity
- Pre-activated, ready-to-use
- High binding capacity
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- Antibody has to be first reduced befor
coupling
- Unsuitable for antibodies with low affinity
for their antigens
|
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- Pre-activated and ready-to-use
- Fast, efficient coupling for large-scale
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- Antigen-binding site in some antibodies
may be adversely affected by coupling.
- Precipitation may happen for some
antibodies.
|
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|
- High coupling capacity
- Fast, efficient coupling for large-scale
|
|
- Antigen-binding site in some antibodies
may be adversely affected by coupling.
- Precipitation may happen for some
antibodies.
|
|
|
- High coupling capacity
- Fast, efficient coupling for large-scale
|
|
- Antigen-binding site in some antibodies
may be adversely affected by coupling.
- Precipitation may happen for some
antibodies .
|
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|
- Antibodies keep Correct orientation for antibody to bind
antigen
- Very gentle binding conditions
- Good choice for high affinity antibodies
- Gentle elution conditions
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