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Human E3 ubiquitin-protein ligase RNF5 (a.a.1-180)
RNF5 Swiss Prot#
Q99942
RNF5 Protein names
E3 ubiquitin-protein ligase RNF5 (EC 6.3.2.-) (RING finger protein 5) (Ram1

homolog) (HsRma1) (Protein G16)
RNF5 Gene names
RNF5 G16 NG2 RMA1
Organism
Human
RNF5 Entry name
RNF5_HUMAN
RNF5 Codon optimized cDNA Clone
RNF5 CDNA Cat#
RPS0729
cDNA Tag
cDNA RNF5 Contains A 6x histidine tag
Condon optimized RNF5 CDNA Formulation
Liquid
Condon optimized RNF5 CDNA Storage
Ship at Room temperature. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw
Condon optimized RNF5 cDNA Applications
Applications: 1. Codon-optimized cDNA sequence designed for recombinant

protein production in both E.coli and Mammalian cell. The cDNA has been

cloned into E.coli expression vector with 6x Histidine tag.2. May be used as

RNAi assistance to restore the loss-of-function.

Recombinant RNF5 Protein

Recombinant RNF5 Protein Cat#
PS0729
RNF5 Protein Source
Recombinant protein.
Recombinant RNF5 Protein Applications
Western Blot, may be used for other applications determined by user
Recombinant RNF5 Protein Purity
>90%, as determined by SDS-PAGE under reducing conditions
Recombinant RNF5 Protein Activity
N/A
Recombinant protein Tag
Recombinant RNF5 contains A 6x histidine tag
Recombinant RNF5 Protein Formulation
Liquid
Recombinant RNF5 Protein Storage
Ship at 4℃. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw
Place Order for Recombinant RNF5 Protein or cDNA
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RNF5 Protein family
-
RNF5 Subcellular locations
Membrane; Multi-pass membrane protein. Mitochondrion membrane.

Endoplasmic reticulum membrane.
RNF5 FUNCTION (According to UniProtKB/Swiss Prot)
FUNCTION: Has E2-dependent E3 ubiquitin-protein ligase activity. May

function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A

and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella

type III secreted protein sopA. May be involved in regulation of cell motility

and localization of PXN/paxillin. Mediates the 'Lys-63'-linked

polyubiquitination of JKAMP thereby regulating JKAMP function by

decreasing its association with components of the proteasome and ERAD;

the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme

UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-

150' leading to its proteasomal degradation; the ubiquitination occurrs in

mitochondria after viral transfection and regulates antiviral responses.
RNF5 Sequence databases
EMBL DDBJ GenBank
RNF5 3D structure databases
ModBase
RNF5 Protein-protein interaction databases
STRING
RNF5 Proteomic databases
PRIDE
RNF5 Genome annotation databases
Ensembl GeneID KEGG UCSC
RNF5 Organism specific databases
CTD MGI
RNF5 Phylogenomic databases
eggNOG GeneTree HOGENOM HOVERGEN

InParanoid OrthoDB PhylomeDB OMA
RNF5 Gene expression databases
Bgee ArrayExpress Genevestigator
RNF5 Family and domain databases
InterPro PROSITE ProtoNet
FOR in vitro RESEARCH USE ONLY