BcMag™ Hydroxyapatite-Modified Magnetic Particles are silica-coated superparamagnetic beads modified with Hydroxyapatite functional groups on the surface. The beads are extremely useful chromatomedium for quick purification and fractionation of a variety of biological substances, such as proteins and antibodies without the need for laborious repeat of pipetting and centrifugation. Hydroxyapatite has two different adsorbing sites (Fig.1); a calcium site that binds with the acidic groups and a phosphate site that interacts with basic protein groups. Thanks to the unique mixed-mode ion exchange characteristics, hydroxyapatite does not only have unique separation properties, unparalleled selectivity and resolution, but also have a very large binding capacity for proteins and antibodies. Furthermore, hydroxyapatite does not bind free amino acids or small peptides, making it advantageous for protein purification.
Features and Advantages
Quick, Easy and one-step high-throughput procedure; eliminates columns
or filters, or laborious repeat of pipetting or centrifugation (Fig.1)
Extremely high binding capacity
Scalable - easily adjusts for sample size and automation